Cyclic Nucleotide-induced Maturation

ment and increased linearly for 72 h. Receptor expression was dependent on new protein synthesis. At 48 h, Scatchard analysis demonstrated 2.4 X 105 receptors/ cell with a KD of 1.3 nM. In contrast to induction of HL60 differentiation by dimethyl sulfoxide, retinoic acid, or phorbol myristate acetate, the developmental programs initiated by agents that raised intracellular adenosine 3'5'-cyclic monophosphate shared several unique features: (a) plasma membrane maturation was dissociated from morphological maturation; (b) no latent period was evident following induction-the earliest membrane Address correspondence to Dr. Chaplinski. Received for publication 17 May 1982 and in revised form 9 August 1982. marker was expressed within 2 h; (c) commitment to terminal differentiation was delayed.